Lub sij hawm ntsuam xyuas tau txo qis los ntawm kwv yees li ib teev mus rau ob peb feeb los ntawm txoj kev qhia tshiab RTF-EXPAR uas siv rov qab transcriptase-free (RTF) mus kom ze rau kev hloov pauv ntawm RNA mus rau hauv DNA ua raws li EXPAR (Exponential Amplification Reaction) rau kev nthuav dav ntawm qhov kub thiab txias.
Tswj tus nqi ntawm Covid-19 Kev sib kis yuav tsum muaj qhov tseeb thiab nrawm dua qhov ntsuas tus kab mob. RT-PCR (rov qab transcriptase polymerase saw cov tshuaj tiv thaiv), txoj kev ntsuas qhov tseeb tshaj plaws uas tau ua haujlwm tam sim no yog kev sim ob-kauj ruam uas siv sijhawm li 60 feeb rau ib tus qauv.
Cov kws tshawb fawb los ntawm University of Birmingham tau tshaj tawm txoj hauv kev tshiab rau kev kuaj pom ntawm SARS-CoV-2. Qhov no tuaj yeem ua rau muaj kev sim sai dua thiab muaj kev nkag siab txaus.
Kev kuaj pom tus kab mob RNA los ntawm RT-PCR (rov qab transcriptase polymerase saw cov tshuaj tiv thaiv) suav nrog kev hloov pauv ntawm tus kab mob RNA rau ntxiv DNA (cDNA) ua raws li kev nthuav dav ntawm cDNA los ntawm ntau PCR (qPCR). Tom qab ntawd, cDNA raug kuaj pom siv cov xim fluorescent. Qhov no siv sijhawm txog li ib teev.
Lub sij hawm ntsuam xyuas tau txo qis los ntawm kwv yees li ib teev mus rau ob peb feeb los ntawm txoj kev qhia tshiab RTF-EXPAR uas siv rov qab transcriptase-free (RTF) mus kom ze rau kev hloov pauv ntawm RNA rau hauv DNA ua raws li EXPAR (Exponential Amplification Reaction) rau amplification ntawm ib qho kub. Lub amplification noj qhov chaw ntawm ib qhov kub thiab txias yog tus yuam sij rau kev ceev, vim hais tias nws tsis txhob ntev cua sov thiab txias cov kauj ruam ntawm RT-PCR. Tsis tas li ntawd, ntu ntawm DNA tau nthuav dav me dua piv rau RT-PCR. Li no, EXPAR tsim txog 108 cov khoom ntawm DNA hauv ob peb feeb. Lub duplex tsim yog saib xyuas, xws li hauv RT-PCR txoj kev siv fluorescent dye, SYBR Green.
Qhov zoo siab, txoj kev tshiab tuaj yeem hloov kho kom pom ntau yam kab mob sib kis los ntawm cov kab mob RNA, piv txwv li Ebola, RSV, thiab lwm yam.
Qhov (s):
Carter et al (2020). Sub-5-feeb Kev Ntsuas ntawm SARS-CoV-2 RNA siv Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR. Ua ntej. Luam tawm ntawm medRxiv Tshaj tawm Lub Ib Hlis 04, 2021. DOI: https://doi.org/10.1101/2020.12.31.20248236
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Lus raug kaw.